Cataloguenumber
CYT-921
Synonyms
GrowthHormoneReceptor,GrowthHormoneBindingProtein,SomatotropinReceptorGHReceptor,SerumBindingProtein,GHBP,GHIP.
Introduction
Growthhormonereceptor,alsoknownasGHRispartofthecytokinereceptorfamily.GHRbinds2receptormoleculesandinthismannerinducessignaltransductionthroughreceptordimerization.Fur
Thermore,athighconcentrations,growthhormoneperformsasanantago
NISTsincethereisalargedifferenceinaffinitiesattherespectivebindingsites.Theantagonistactioncanbeenhancedfurtherbyreducingbindinginthelowaffinitybindingsite.
Description
GHBPproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining254aminoacids(19-264a.a.)andhavingamolecularmassof29.4kDa(MolecularsizeonSDS-PAGEwillappearatapproximately28-40kDa).GHBPisexpressedwithan8aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
GHBPproteinsolution(1mg/ml)containsphosphatebufferedsaline(pH7.4)and10%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
FSGSEATAAILSRAPWSLQSVNPG
LKTNSSKEPKFTKCRSPERETFSCHWTDEVHHGTKNLGPIQLFYTRRNTQEWTQEWKECPDYVSAGENSCYFNSSFTSIWIPYCIKLTSNGGTVDEKCFSVDEIVQPDPPIALNWTLLNVSLTGIH
ADIQVRWEAPRNADIQKGWMVLEYELQYKEVNETKWKMMDPILTTSVPVYSLKVDKEYEVRVRSKQRNSGNYGEFSEVLYVTLPQMSQFTCEEDFY
LEHHHHHH.
SafetyDataSheet
Usage
ProspecsproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
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