Cataloguenumber
PRO-2076
Synonyms
Elongationfactor2,EF-2,EEF2,EF2,EukaryoticTranslationElongationFactor2,EEF-2.
Introduction
EukaryoticTranslationElongationFactor2(EEF2)whichisnecessaryfactorforproteinsynthesisisapartoftheGTP-bindingtranslationelongationfactorfamily.EEF2catalyzestheGTP-dependentribosomaltranslocationstepduringtranslationelongation.EEF2isalsocatalyzesthecoordinatedmovementofthemRNAaswellasthe2tRNAmoleculesandconformationalchangesintheribosome.EF-2kinasephosporylationinactivatestheEEF2protein.
Description
EEF2HumanRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining308aminoacids(574-858)andhavingamolecularmassof34.3kDa.
EEF2isfusedtoa23aminoacidHis-TagatN-terminusandpurifiedbyproprietarychromatographictechniques.
Source
E.coli.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
TheEEF2solution(1mg/ml)contains20mMTris-HClbuffer(pH8.0),10%glyceroland0.4MUrea.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.