PRO-2477
Ephrin-A1,EPLG1,TNFAIP4,LERK1,EFL1,ECKLG,EPH-relatedreceptortyrosinekinaseligand1,ImmediateearlyresponseproteinB61,Tumornecrosisfactoralpha-inducedprotein4,TNFalpha-inducedprotein4,ligandofeph-relatedkinase1,tumornecrosisfactor,alpha-inducedprotein4.
EFNA1belongstotheephrin(EPH)family.TheEPHsubfamilyisthebiggestgroupofreceptorproteinkinasesandtheytakepartinvitalnervoussystemfunctionanddevelopment.
EFNA1HumanRecombinantproducedinHEK293cellsisasingle,glycosylatedpolypeptidechain(a.a19-182)containing170aminoacidsincludinga6a.aC-terminalHistag.Thetotalmolecularmassis20.2kDa(calculated).
HEK293cells.
EFNA1filtered(0.4µm)andlyophilizedfrom0.5mg/mlinphosphatebufferedsaline,pH7.5containing5%(w/v)trehalose.
Itisrecommendedtoadd200µldeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.EFNA1isnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Storelyophilizedproteinat-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcycles.Reconstitutedproteincanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeafteroneweekat4°C.
Greaterthan95.0%asdeterminedbySDS-PAGE.
DRHTVFWNSSNPKFRNEDYTIHVQLNDYVDIICPHYEDHSVADAAMEQYILYLVEHEEYQLCQPQSKDQVRWQCNRPSAKHGPEKLSEKFQRFTPFTLGKEFKEGHSYYYISKPIHQHEDRCLRLKVTVSGKITHSPQAHDNPQEKRLAADDPEVRVLHSIGHSHHHHHH.
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。