Cataloguenumber
PRO-2344
Synonyms
Ephrintype-Breceptor4(EC:2.7.10.1),Developmentalkinase2,mDK-2,Hepatomatransmembranekinase,TyrosinekinaseMYK-1,Ephb4,Htk,Mdk2,Myk1.
Introduction
Ephrintype-Breceptor4isoformb(Ephb4)belongstotheEphreceptortyrosinekinasefamilywhichfunctionsinneuronalguidanceandmediatevenal/arterialseparation.EPHB4-mediatedforwardsignalingregulatescellularrepulsionandsegregationformEFNB2-expressingcells.Fur
Thermore,Ephb4hasaroleinpostnatalbloodvesselremodeling,morphogenesisandperme
ABIlityandisthereforeessentialinthecontextoftumorangiogenesis.
Description
EPHB4MouseRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining532aminoacids(16-539a.a.)andhavingamolecularmassof58.7kDa(Migratesat50-70kDaonSDS-PAGEunderreducingconditions).
EPHB4isexpressedwithan8aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
SterileFilteredclearsolution.
Formulation
EPHB4proteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
Stability
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85.0%asdeterminedbyanalysisbySDS-PAGE.
Aminoacidsequence
LEETLLNTKLETADLKWVTYPQAEGQWEELSGLDEEQHSVRTYEVCDMKRPGGQAHWLRT
GWVPRRGAVHVYATIRFTMMECLSLPRASRSCKETFTVFYYESEADTATAHTPAWMENPY
IKVDTVAAEHLTRKRPGAEATGKVNIKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYK
KCSWLITNLTYFPETVPRELVVPVAGSCVANAVPTANPSPSLYCREDGQWAEQQVTGCSC
APGYEAAESNKVCRACGQGTFKPQIGDESCLPCPANSHSNNIGSPVCLCRIGYYRARSDP
RSSPCTTPPSAPRSVVHHLNGSTLRLEWSAPLESGGREDLTYAVRCRECRPGGSCLPCGG
DMTFDPGPRDLVEPWVAIRGLRPDVTYTFEVAALNGVSTLATGPPPFEPVNVTTDREVPP
AVSDIRVTRSSPSSLILSWAIPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELR
GLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESESWREQLAVEHHHHHH. Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.