Cataloguenumber
ENZ-654
Synonyms
Polymerase(RNA)II(DNADirected)PolypeptideJ3,DNA-DirectedRNAPolymeraseIISubunit11,DNA-DirectedRNAPolymeraseIISubunitRPB11-B2,DNA-DirectedRNAPolymeraseIISubunitJ3,RNAPolymeraseIISubunitB11-B2,POLR2J2,RPB11b2,RPB11b1.
Introduction
DNA-directedRNApolymeraseIIsubunitRPB11-b2(POLR2J3)isamemberoftheeukaryoticRPB11RNApolymerasesubunitfamily.POLR2J(DNA-directedRNApolymeraseIIsubunitJ)existsin3variantsPOLR2J1(RPB11-a),POLR2J2(RPB11-b1),andPOLR2J3(RPB11-b2).POLR2J3isaDNA-dependentRNApolymerasewhichcatalyzesthetranscriptionofDNAintoRNAusingthefourribonucleosidetriphosphatesassubstrates.
Description
POLR2J3HumanRecombinantproducedinE.coliisasinglepolypeptidechaincontaining138aminoacids(1-115)andhavingamolecularmassof15.5kDa.
POLR2J3isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
E.coli.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
ThePOLR2J3solution(0.5mg/1ml)contains20mMTris-HClbuffer(pH8.0),0.2MNaCl,1mMDTTand40%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMNAPPAFESFLLFEGEKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRTCLLPLRLLP
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.