Cataloguenumber
ENZ-745
Synonyms
Galactoside3(4)-L-fucosyltransferase,BloodgroupLewisalpha-4-fucosyltransferase,LewisFT,Fucosyltransferase3,FucosyltransferaseIII,FucT-III,FUT3,FT3B,LE,CD174,Les.
Introduction
Fucosyltransferase3(FUT3)catalyzesalpha-1,3andalpha-1,4glycosidiclinkageswhichtakepartintheexpressionofVim-2,LewisA,LewisB,sialylLewisXandLewisX/SSEA-1antigens.FUT3takespartinbloodgroupLewisdetermination;Lewis-positive(Le+)individualshaveanactiveenzymewhileLewis-negative(Le-)individualshaveaninactiveenzyme.FUT3alsooperatesonthecorresponding1,4-galactosylderivative,creating1,3-L-fucosyllinks.
Description
FUT3HumanRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining350aminoacids(35-361a.a)andhavingamolecularmassof40.6kDa.
FUT3isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredclearsolution.
Formulation
TheFUT3solution(1mg/ml)contains20mMTris-HClbuffer(pH8.0),0.4Mureaand10%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan80%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSRVSRDDATGSPRAPSGSSRQDTTPTRPTLLILLWTWPFHIPVALSRCSEMVPGTADCHITADRKVYPQADTVIVHHWDIMSNPKSRLPPSPRPQGQRWIWFNLEPPPNCQHLEALDRYFNLTMSYRSDSDIFTPYGWLEPWSGQPAHPPLNLSAKTELVAWAVSNWKPDSARVRYYQSLQAHLKVDVYGRSHKPLPKGTMMETLSRYKFYLAFENSLHPDYITEKLWRNALEAWAVPVVLGPSRSNYERFLPPDAFIHVDDFQSPKDLARYLQELDKDHARYLSYFRWRETLRPRSFSWALDFCKACWKLQQESRYQTVRSIAAWFT. Usage
ProspecsproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.