Cataloguenumber
ENZ-671
Synonyms
bA379J5.3,bA555E18.1,C20orf88,DUEB,HARS2,pqn-68,D-tyrosyl-tRNA(Tyr)deacylase1,DNA-unwindingelement-bindingproteinB.
Introduction
D-Tyrosyl-tRNADeacylase1(DTD1)isamemberoftheDTDfamily.DTD1hydrolyzesD-tyrosyl-tRNA(Tyr)intoD-tyrosineandfreetRNA(Tyr).DTD1maybeadefensemechanismagainstadamagingeffectofD-tyrosine.DTD1isanATPaseinvolvedinDNAreplication,whichmayfacilitatelo
ADIngofCDC45ontopre-replicationcomplexes.TheDTD1proteinlocalizestotheDUE(DNAunwindingelements)ofactivereplicationorigins.DTD1isexpressedinnumerousadultandfetaltissues,withthehighestlevelsinthetestis,ovary,spleenandintheadultandfetalbrain.DTD1mightbeariskfactorforAIA(aspirin-intolerantasthma)bycatalyzingthehydrolysisofD-tryptophanandinteractingwiththetyrosyl-tRNAsynthetase(tyrRS)enzymethatpromotesapro-inflammatoryphenotype.
Description
DTD1HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining232aminoacids(1-209a.a.)andhavingamolecularmassof25.9kDa.
DTD1isfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredclearsolution.
Formulation
DTD1proteinsolution(0.5mg/ml)contains20mMTris-HClbuffer(pH8.0),0.1MNaCl,10%glyceroland1mMDTT.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleaseavoidfreezethawcycles.
Purity
Greaterthan85%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMKAVVQRVTRASVTVGGEQISAIGRGICVLLGISLEDTQKELEHMVRKILNLRVFEDESGKHWSKSVMDKQYEILCVSQFTLQCVLKGNKPDFHLAMPTEQAEGFYNSFLEQLRKTYRPELIKDGKFGAYMQVHIQNDGPVTIELESPAPGTATSDPKQLSKLEKQQQRKEKTRAKGPSESSKERNTPRKEDRSASSGAEGDVSSEREP.
Usage
ProspecsproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.