Cataloguenumber
ENZ-1016
Introduction
Chymotrypsinogen-B1(CTRB1)belongstotheserineproteasefamilyofenzymesandformsamainprecursorofthepancreaticproteolyticenzymes.CTRB1islocatednexttoarelatedchymotrypsinogengene.CTRB1isaproteincodinggenewhichencodesdifferentisoformswhichmayundergosimilarprocessingtogeneratethematureprotein.
Description
RecombinantHumanCTRB1expressedinE.colicontaining245aminoacidshavingaMwof27kDaispurifiedbystandardchromatographytechniques.
Source
E.coli
PhysicalAppearance
SterileFilteredlyophilizedpowder.
Formulation
TheHumanCTRB1waslyophilizedwithoutanyadditives.
Solubility
ItisrecommendedtoreconstitutethelyophilizedHumanCTRB1in1ml50mMHAcwhichcanthenbefurtherdilutedtootheraqueoussolutions.
RecombinantHumanCTRB1althoughstableatroomtempfor1week,shouldbestoreddesiccatedbelow-18°C.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95%asdeterminedbyHPLC.
1100units/mgprotein.
Oneunitisdefinedastheamountofenzymethatwillhydrolyze1.0μmoleofN-alpha-acetyl-L-tyrosineethylester(ATEE)perminatpH7.0at25°C.
Aminoacidsequence
CGVPAIHPVLSGLSRIVNGEDAVPGSWPWQVSLQDKTGFHFCGGSLISEDWVVTAAHCGVRTSDVVVAGEFDQGSDEENIQVLKIAKVFKNPKFSILTVNNDITLLKLATPARFSQTVSAVCLPSADDDFPAGTLCATTGWGKTKYNANKTPDKLQQAALPLLSNAECKKSWGRRITDVMICAGASGVSSCMGDSGGPLVCQKDGAWTLVGIVSWGSDT
CSTSSPGVYARVTKLIPWVQKILAAN.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.