Carboxymethyle
NEButenolidasehomolog,CMBL,JS-1.
Carboxymethylenebutenolidasehomolog(CMBL)isacysteinehydrolaseofthedienelactonehydrolasefamilywhichishighlyexpressedinthelivercytosol.CMBListhehumanhomologofPseudomonasdienelactonehydrolase,whichisaproteinthatparticipatesinthebacterialhalocatecholdegradationpathway.CMBLwhichpreferentiallycleavescyclicestersactivatesmedoxomil-esterprodrugsinwhichthemedoxomilmoietyiscoupledwithanoxygenatom.CMBLisinhibitedbyPCMB(p-chloromercuribenzoate)andisencodedbyagenewhichmapstohumanchromosome5p15.2.Fur
Thermore,CMBLconvertstheprodrugolmesartanmedoxomilintoitspharmacologicallyactivemetaboliteolmerstatan,whichisanangiotensinreceptorblocker,intheliverandintestine.CMBLcanalsoactivatebeta-lactamantibioticsfaropenemmedoxomilandlenampicillin.CMBLiswidelyexpressed,withthehighestlevelsintheliver,followedbythekidney,smallintestineandthecolon.
CMBLHumanRecombinantproducedinE.coliisasinglepolypeptidechaincontaining269aminoacids(1-245)andhavingamolecularmassof30.6kDa.
CMBLisfusedtoa24aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
EscherichiaColi.
Sterilefilteredcolorlesssolution.
TheCMBLsolution(0.5mg/ml)contains20mMTris-HClbuffer(pH8.0),0.1MNaCl,10%glyceroland1mMEDTA.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Greaterthan95.0%asdeterminedbySDS-PAGE.
Prospec"sproductsarefurnishedfor LABORATORYRESEARCH USE ONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.