Cataloguenumber
ENZ-572
Synonyms
Aspartoacylase,Aminoacylase-2,ACY-2,ASPA,ACY2,ASP.
Introduction
AspartoacylaseisahomodimerwhichcatalyzesthedeacetylationofN-acetylasparticacid(NAA)(aproteinwhosehydrolysisiscrucialtomaintenanceofintactwhitematter)togenerateacetateandL-aspartate.Aspartoacylase(ASPA)isexpressedintheliver,lungandkidneytissue,aswellasintheskeletalmuscleandincerebralwhitematter.NAAisampleinthebrainwherehydrolysisbyaspartoacylaseisbelievedtoaidmaintainwhitematter.InothertissuesASPAfunctionsasascavengerofNAAfrombodyfluids.ASPAgenemutationscauseCanavandisease(CANDorspongydegenerationofthebrain).
Description
ASPAHumanRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining336aminoacids(1-313)andhavingamolecularmassof38.1kDa.
ASPAisfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredclearsolution.
Formulation
TheASPAsolution(0.5mg/ml)contains20mMTris-HClbuffer(pH8.0),20%glycerol,1mMDTT,0.1MNaCland0.1mMPMSF.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMTSCHIAEEHIQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRYIDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILEDSRNNFLIQMFHYIKTSLAPLPCYVYLI
EHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCAIEVYKIIEKVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTTKLTLNAKSIRCCLH. Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.