Prospec/FLT1 D5 Human/PKA-239/100µg,Prospec,,Prospec,prospecbio,ProSpec-Tany,Prospec代理，产品目录进口prospecbio试剂盒，血清清关,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Prospec/FLT1 D5 Human/PKA-239/100µg

产品编号：PKA-239

市场价：¥19800.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>生长因子>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$990.00

品牌： Prospec

公司：Prospec-Tany

公司分类：

商品介绍

Cataloguenumber

PKA-239

Synonyms

FLT-1,FLT1,Tyrosine-proteinkinasereceptorFLT,Flt-1,Tyrosine-proteinkinaseFRT,Fms-liketyrosinekinase1,VEGFR-1.

Introduction

Endothelialcellsexpressthreedifferentvascularendothelialgrowthfactor(VEGF)receptors,belongingtothefamilyofreceptortyrosinekinases(RTKs).TheyarenamedVEGFR-1(Flt-1),VEGFR-2(KDR/Flk-1),VEGFR-3(Flt-4).Theirexpressionisalmostexclusivelyrestrictedtoendothelialcells,butVEGFR-1canalsobefoundonmonocytes,dendriticcellsandontrophoblastcells.Theflt-1genewasfirstdescribedin1990.Thereceptorcontainssevenimmunoglobulin-likeextracellulardomains,asingletransmembraneregionandanintracellularsplitedtyrosinekinasedomain.ComparedtoVEGFR-2theFlt-1receptorhasahigheraffinityforVEGFbutaweakersignalingactivity.VEGFR-1thusleadsnottoproliferationofendothelialcells,butmediatessignalsfordifferentiation.InterestinglyanaturallyoccuringsolublevariantofVEGFR-1(sVEGFR-1)wasfoundinHUVEsupernatantsin1996,whichisgeneratedbyalternativesplicingoftheflt-1mRNA.TheBIOLOGicalfunctionsofsVEGFR-1stillarenotclear,butitseemstobeanendogenousregulatorofangiogenesis,bindingVEGFwiththesameaffinityasthefull-lengthreceptor.

Description

SolubleFLT1D1-5HumanRecombinantproducedinbaculovirusismonomeric,glycosylated,polypeptidecontaining562aminoacidsandhavingamolecularmassof70kDa.Thesolublereceptorproteincontainsonlythefirst5extracellulardomains,whichcontainalltheinformationnecessaryforbindingofVEGF.
TheFLT1ispurifiedbyproprietarychromatographictechniques.

Source

InsectCells.

PhysicalAppearance

SterileFilteredWhitelyophilized(freeze-dried)powder.

Formulation

FLT1D1-5waslyophilizedfromaconcentrated(1mg/ml)sterilesolutioncontainingnoadditives.

Solubility

ItisrecommendedtoreconstitutethelyophilizedFLT1D5insterilewaternotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.

StABIlity

LyophilizedFLT-1althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionFLT1shouldbestoredat4°Cbetween2-7daysandforfutureusebelow
-18°C.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.

Purity

Greaterthan90.0%asdeterminedby(a)AnalysisbyRP-HPLC.
(b)AnalysisbySDS-PAGE.

BiologicalActivity

TheactivityofFLT1D5wasdeterminedbyitsabilitytoabolishthebindingofiodinatedVEGFtosolidsurfacesorcellsurfaces.TheED50forthiseffectistypically10ng/ml,correspondingtoaspecificactivityof100,000IU/mg.
Ina13dayCAM-assaysVEGFR-1isabletoinhibitVEGFstimulatedsproutingofcapillariesat30pM.

Usage

Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.

品牌介绍

ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。