FLT-1,FLT1,Tyrosine-proteinkinasereceptorFLT,Flt-1,Tyrosine-proteinkinaseFRT,Fms-liketyrosinekinase1,VEGFR-1.
Endothelialcellsexpressthreedifferentvascularendothelialgrowthfactor(VEGF)receptors,belongingtothefamilyofreceptortyrosinekinases(RTKs).TheyarenamedVEGFR-1(Flt-1),VEGFR-2(KDR/Flk-1),VEGFR-3(Flt-4).Theirexpressionisalmostexclusivelyrestrictedtoendothelialcells,butVEGFR-1canalsobefoundonmonocytes,dendriticcellsandontrophoblastcells.Theflt-1genewasfirstdescribedin1990.Thereceptorcontainssevenimmunoglobulin-likeextracellulardomains,asingletransmembraneregionandanintracellularsplitedtyrosinekinasedomain.ComparedtoVEGFR-2theFlt-1receptorhasahigheraffinityforVEGFbutaweakersignalingactivity.VEGFR-1thusleadsnottoproliferationofendothelialcells,butmediatessignalsfordifferentiation.InterestinglyanaturallyoccuringsolublevariantofVEGFR-1(sVEGFR-1)wasfoundinHUVEsupernatantsin1996,whichisgeneratedbyalternativesplicingoftheflt-1mRNA.The
BIOLOGicalfunctionsofsVEGFR-1stillarenotclear,butitseemstobeanendogenousregulatorofangiogenesis,bindingVEGFwiththesameaffinityasthefull-lengthreceptor.
FLT1D1-3HumanRecombinantproducedinbaculovirusismonomeric,glycosylated,polypeptidecontaining298aminoacidsfragment(31-328)andhavingamolecularmassof38.16kDa.Thereceptorproteincontainsonlythefirst3extracellulardomains,whichcontainalltheinformationnecessaryforbindingofVEGF.
TheFLT1ispurifiedbyproprietarychromatographictechniques.
InsectCells.
SterileFilteredclearsolution.
FLT1His(0.96mg/ml)issuppliedin25mMNa-AcetatepH4.8and50%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Pleaseavoidfreezethawcycles.
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.