Cataloguenumber
PRO-744
Synonyms
Eosinophillysophospholipase,Charcot-Leydencrystalprotein,CLC,Galectin-10,Gal-10,Lysolecithinacylhydrolase,GAL10,LGALS10,LGALS10A.
Introduction
Eosinophillysophospholipase(CLC)actson
BIOLOGicalmembranestoregulatethemultifunctionallysophospholipids.CLCisalysophospholipaseexpressedineosinophilsandbasophils.CLChydrolyzeslysophosphatidylcholinetoglycerophosphocholineandafreefattyacid.TheCLCproteinmaypossesscarbohydrateorIgE-bindingactivities.CLCisbothstructurallyandfunctionallyrelatedtothegalectinfamilyofbeta-galactosidebindingproteins.CLCmaybelinkedwithinflammationandsomemyeloidleukemias.
Description
LGALS10producedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining162aminoacids(1-142a.a.)andhavingamolecularmassof18.6kDa.
LGALS10isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
Galectin-10proteinsolution(0.5mg/ml)containing20mMTris-HClbuffer(pH8.0),1mMDTT,10%glyceroland0.1MNaCl.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMSLLPVPYTEAASLSTGSTVTIKGRPLACFLNEPYLQVDFHTEMKEESDIVFHFQVCFGRRVVMNSREYGAWKQQVESKNMPFQDGQEFELSISVLPDKYQVMVNGQSSYTFDHRIKPEAVKMVQVWRDISLTKFNVSYLKR.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.