Cataloguenumber
CYT-861
Synonyms
ProteinWnt-3a,MGC119418,MGC119419,MGC119420,WNT3A,wingless-typeMMTVintegrationsitefamilymember3A.
Introduction
TheWNTgenefamilyconsistsofstructurallyrelatedgenesthatencodesecretedsignalingproteins.Theseproteinsareimplicatedinoncogenesisandinseveraldevelopmentalprocesses,includingregulationofcellfateandpatterningduringembryogenesis.WNT3AisamemberoftheWNTgenefamily.WNT3Aisaproteinwhichshows96%aminoacididentitytomouseWnt3Aprotein,and84%tohumanWNT3protein,anotherWNTgeneproduct.TheWNT3AgeneisclusteredwithWNT14gene,anotherfamilymember,inchromosome1q42region.
Description
WNT3AHumanRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining335aminoacids(19-352a.a.)andhavingamolecularmassof37.5kDa.TheWNT3AHumanispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredclearsolution.
Formulation
TheWNT3AHumanproteinsolution(0.5mg/ml)contains20mMTris-HClbuffer(pH8.0)and10%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MSYPIWWSLAVGPQYSSLGSQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGIKIGIQECQHQFRGRRWNCTTVHDSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGTAAICGCSSRHQGSPGKGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRAIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARAERRREKCRCVFHWCCYVSCQECTRVYDVHTCK.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.