Cataloguenumber
CYT-752
Synonyms
Uteroglobin,Claracellphospholipid-bindingprotein,CCPBP,Claracells10kDasecretoryprotein,CC10,Secretoglobinfamily1Amember1,Urinaryprotein1,UP-1,UP1,Urineprotein1,SCGB1A1,CCSP,UGB,CC16.
Introduction
Uteroglobin(SCGB1A1)whichbelongstotheSecretoglobin(SCGBs)superfamily,isamultifunctionalproteinthatexertsanti-inflammatoryandanti-tum
Origeniceffectsbybindingsmallhydrophobicmoleculessuchasphospholipidsandprostaglandins.Uteroglobinisinvolvedinnumerousfunctionsincludinganti-inflammation,inhibitionofphospholipaseA2andthesequesteringofhydrophobicligands.SCGB1A1isexpressedbyClaracells,thenon-ciliated,non-mucoussecretorycellspredominantinlungbronchioles,andbyotherepitheliawhichcommunicatewiththeexternalenvironment.Ontopofsequesteringpro-inflammatorymediatorsandcarcinogens,Uteroglobinisimplicatedintheinhibitionofcellmigrationandinvasion,plateletaggregation,andTcelldifferentiation.SCGB1A1genedefectsareassociatedwithasusceptibilitytoasthma.
Description
UteroglobinHumanRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining80aminoacidsandincludinga10a.aN-terminalHistag. Thetotalmolecularmassis9.2kDa(calculated).
Source
EscherichiaColi.
PhysicalAppearance
FilteredWhitelyophilized(freeze-dried)powder.
Formulation
Filtered(0.4µm)andlyophilizedfrom0.5mg/mlin0.05Mphosphatebufferand0.075MNaCl,pH7.4.
Solubility
Itisrecommendedtoadd200µldeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.SCGB1A1isnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Storelyophilizedproteinat-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcycles.Reconstitutedproteincanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeaftertwoweeksat4°C.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MKHHHHHHASEICPSFQRVIETLLMDTPSSYEAAMELFSPDQDMREAGAQLKKLVDTLPQKPRESIIKLMEKIAQSSLCN.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.