Cataloguenumber
ANT-302
Synonyms
TumorproteinD53,TumorproteinD52-like1,TPD52L1,hD53,D53,MGC8556,TPD52L2.
Type
MouseAntiHumanMonoclonal.
Introduction
TPD52L1(hD53,hD53L1)isamemberofD52genefamilyandoverexpressedinhumanbreastcarcinoma.TPD52L1containscoiled-coilmotif,whichisrespons
IBLeforhomo-andheteromericinteraction,andmaybearegulatorofcellproliferationandcalcium-mediatedsignaltransduction.Moreover,TPD52L1interactswiththemitogen-activatedproteinkinasekinasekinase5(MAP3K5/ASK1)andpositivelyregulatesMAP3K5-inducedapoptosis.
Clone
Pd1C5AT.
Immunogen
Anti-humanTPD52L1mAb,isderivedfromhybridizationofmouseSP2/OmyelomacellswithspleencellsfromBALB/cmiceimmunizedwithrecombinanthumanTPD52L1aminoacids1-131purifiedfromE.coli.
IgSubclass
MouseIgG2bheavychainandκlightchain.
PurificationMethod
TPD52L1antibodywaspurifiedfrommouseasciticfluidsbyprotein-Gaffinitychromatography.
Formulation
1mg/mlcontainingPBS,pH-7.4,&0.1%SodiumAzide.
StorageProcedures
Forperiodsupto1monthstoreat4°C,forlongerperiodsoftime,storeat-20°C.Preventfreezethawcycles.
StABIlity/ShelfLife
12monthsat-20°C.1monthat4°C.
Applications
TPD52L1antibodyhasbeentestedby
ELISA,Westernblotandimmunohistochemistryanalysistoassurespecificity.Sinceapplicationvaries,however,eachinvestigationshouldbetitratedbythereagenttoobtainoptimalresults.RecommendeddilutionrangeforWesternblotanalysisis1:500~1:3,000.Recommendedstartingdilutionis1:1000.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
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