Cataloguenumber
ANT-423
Synonyms
CYR61,ProteinCYR61,Cysteine-richangiogenicinducer61,Insulin-likegrowthfactor-bindingprotein10,IGF-bindingprotein10,IGFBP-10,IBP-10,ProteinGIG1,CCNfamilymember1,CCN1,GIG1,IGFBP10.
Type
MouseAntiHumanMonoclonal.
Introduction
CYR61isagrowthfactor-induc
IBLe,immediate-earlygenethathasmultifacetedactivitiesinvariouscancers.CYR61isasecreted,cysteine-rich,heparin-bindingproteinwhichisencodedbyagrowthfactor-inducibleimmediate-earlygene.Actingasanextracellular,matrix-associatedsignalingmolecule,CYR61promotestheadhesionofendothelialcellsthroughinteractionwithintegrinandenhancesgrowthfactor-inducedDNAsynthesisinthesamecelltype.
Clone
P3H3AT.
Immunogen
Anti-humanCYR61mAb,isderivedfromhybridizationofmouseFOmyelomacellswithspleencellsfromBALB/cmiceimmunizedwithrecombinanthumanCYR61aminoacids168-242purifiedfromE.coli.
IgSubclass
MouseIgG1heavychainandκlightchain.
PurificationMethod
CYR61antibodywaspurifiedfrommouseasciticfluidsbyprotein-Gaffinitychromatography.
PhysicalAppearance
SterileFilteredclearsolution.
Formulation
1mg/mlcontainingPBS,pH-7.4,&0.1%SodiumAzide.
StorageProcedures
Forperiodsupto1monthstoreat4°C,forlongerperiodsoftime,storeat-20°C.Preventfreezethawcycles.
StABIlity/ShelfLife
12monthsat-20°C.1monthat4°C.
Applications
CYR61antibodyhasbeentestedby
ELISA,Westernblotandimmunohistochemistryanalysistoassurespecificityandreactivity.Sinceapplicationvaries,however,eachinvestigationshouldbetitratedbythereagenttoobtainoptimalresults.RecommendeddilutionrangeforWesternblotis1:250~1:1,000andimmunohistochemistryanalysisis1:50~100.RecommendedstartingdilutionforWesternblotis1:250andImmunohistochemistryis1:50.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.