Cataloguenumber
ANT-119
Synonyms
Smallinduc
IBLecytokineA2,CCL2,Monocytechemotacticprotein1,MCP-1,Monocytechemoattractantprotein1,Monocytechemotacticandactivatingfactor,MCAF,MonocytesecretoryproteinJE,HC11,chemokine(C-Cmotif)ligand2,MCP1,SCYA2,GDCF-2,SMC-CF,HSMCR30,MGC9434,GDCF-2HC11.
Type
MouseAntiHumanMonoclonal.
Introduction
Chemokine(C-Cmotif)ligand2(CCL2)isasmallcytokinebelongingtotheCCchemokinefamilythatisalsoknownasmonocytechemotacticprotein-1(MCP-1).Itisfoundatthesiteoftootheruptionandbonedegradation.Inthebone,CCL2isexpressedbymatureosteoclastsandosteoblastsandisunderthecontrolofnuclearfactor?B(NF?B).CCL2recruitsimmunecells,suchasmonocytes,tositesoftissueinjuryandinfection.Thischemokineisproducedasaproteinprecursorcontainingsignalpeptideof23aminoacidsandamaturepeptideof76aminoacids.Itisamonomericpolypeptide,withamolecularweightofapproximately13kDa.AswithmanyotherCCchemokines,CCL2islocatedonchromosome17inhumans.ThecellsurfacereceptorsthatbindCCL2areCCR2andCCR5.
Clone
YNR-HMCAF.
Immunogen
r.HumanMCAF.
IgSubclass
MouseIgG2a.
PurificationMethod
Ionexchange.
Formulation
1mg/mlinPBS(afterreconstitution)ShippingConditionsAntibodyisshippedlyophilizedatambienttemperature.
StorageProcedures
Inlyophilizedform,forlongperiods,storeat4oCinadryenvironment.Afterreconstitution,ifnotintendedforusewithinamonth,aliquotandstoreat-20oC.
Solubility
ReconstitutewithsterileH20.Mixgently,washthesidesofthevialandwait30-60secondsbeforeuse.
Titer
Indirect
ELISA,usingalkalinephosphatasegoatanti-mouseIg(
JacksonLaboratories)1:5,000dilutionwillgive0.3O.Dwithin10minutes.
Applications
DirectELISA,WesternBlot,Immuneprecipitation.
Note
MCAFdoesnotbindtoplasticasgoodasmostproteins-usehigherconcentrationforproperbinding.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.