HumanT-lymphotropicvirus(HTLV)isahuman,single-strandedRNAretrovirusthatcausesT-cellleukemiaandT-celllymphoma.ThevirusactivatesasubsetofT-helpercellscalledTh1cells.TheresultisaproliferationofTh1cellsandoverproductionofTh1relatedcytokines(mainlyIFN-gammaandTNF-alpha).FeedbackmechanismsofthesecytokinescauseasuppressionoftheTh2lymphocytesandareductionofTh2cytokineproduction(mainlyIL-4,IL-5,IL-10andIL-13).Theendresultisareductioninthe
ABIlityoftheinfectedhosttomountanadequateimmuneresponsetoinv
ADIngorganismsthatrequireapredominantlyTh2dependantresponse(theseincludeparasiticinfectionsandproductionofmucosalandhumoralantibodies).
TheE.Coliderivedrecombinantproteincontainsthefull-lengthsequenceofHTLV-Ip24,spanningallofp24.Theproteincontains214aminoacidshavinganMwof24kDa.
HTLV-1p24waspurifiedbyproprietarychromatographictechnique.
HTLV-1p24proteinis>95%pureasdeterminedby10%PAGE(coomassiestaining)andRP-HPLC.
50mMNaPO4pH6.0,containing1mMDTT&1mMEDTA.
HTLV-1p24althoughstableat4°Cfor1week,shouldbestoredbelow-18°C.Pleasepreventfreezethawcycles.
ImmunoreactivewithallseraofHTLV-1infectedindividuals.
HTLV-1p24antigencanbeusedfor
ELISAandWesternblots,excellentantigenforearlydetectionofHIV
SEROconvertorswithminimalspecificityproblems.
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.