Cataloguenumber
PKA-244
Synonyms
Tyrosine-proteinkinasereceptorFLT4,PCL,FLT41,FMS-LIKETYROSINEKINASE4,VEGFR-3,VEGFR3.
Introduction
AllthreeVEGFreceptorsbelongtotheclassIIIsubfamilyofreceptortyrosinekinases(RTKs)characterisedbythesevenimmunoglobulin-likeloopsintheextracellulardomain.TheexpressionofVEGFR-1to-3isalmostexclusivelyrestrictedtohematopoieticprecursorcells,vascularandlymphaticendothelialcellsandtothemonocyte/macrophagelineage.Theyplaykeyrolesinvasculogenesis,hematopoiesis,angiogenesisandlymphangiogenesis.TheFLT-4
CDNAencodesa1298aminoacid(aa)residueprecursorproteinwitha23aaresiduesignalpeptide.MatureVEGFR-3/FLT-4iscomposedofa751aaresidueextracellulardomain,a22aatransmembranedomainanda482aaresiduecytoplasmicdomain.BothVEGFfamilymembersVEGF-CandVEGF-DhavebeenshowntobindandactivateVEGFR-3/FLT-4.TheFlt-4geneiswidelyexpressedintheearlyembryobutbecomesrestrictedtothelymphaticendothelialalatterstagesofdevelopment.Itisimportantforlymphangiogenesis.
Description
SolubleFLT4HumanRecombinantfusedwithacarboxy-terminal6Xhistidine-tagproducedinbaculovirusisamonomeric,glycosylated,polypeptidecontainingtheextracellularpart,25-774aminoacidsandhavingatotalmolecularmassof120kDa.Thesolublereceptorproteincontainsonlythefirst7extracellulardomains,whichcontainalltheinformationnecessaryforligandbinding.TheFLT4ispurifiedbyproprietarychromatographictechniques.
Source
InsectCells.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
FLT4waslyophilizedfromaconcentrated(1mg/ml)sterilesolutioncontaining1xPBS.
Solubility
ItisrecommendedtoreconstitutethelyophilizedFLT4insterilewaternotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedFLT4althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionFLT4shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
MeasuredbyitsabilitytobindrecombinantratVEGF-Cinafunctionalsolidphasebindingassay.ImmobilisedrecombinanthumanVEGFR-3/FLT-4at5µg/mlcanbindrecombinantratVEGF-Cinalinearrangeof8-500ng/ml.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.