Cataloguenumber
ENZ-971
Synonyms
hK2,KLK2A2,Kallikrein-2,Glandularkallikrein-1,hGK-1,issuekallikrein-2,KLK2.
Introduction
KLK2isapartofthegrandularkallikreinproteinfamilywhoseMembersareengagedinadiversearrayof
BIOLOGicalfunctions.Kallikreinsareasubgroupofserineproteaseswhichareclusteredonchromosome19.KLK2isahighlyactivetrypsin-likeserineproteasewhichselectivelycleavesatarginineremains.KLK2ismostlyexpressedinprostatictissueandisaccountableforcleavingpro-prostate-specificantigenintoitsenzymaticallyactiveform.KLK2isgreatlyexpressedinprostatetumorcellsandmayposs
IBLybeaprognosticmakerforprostatecancerrisk.
Description
KLK2HumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining246aminoacids(25-261aa)andhavingamolecularmassof27.2kDa(Migratesat28-40kDaonSDS-PAGEunderreducingconditions).KLK2isexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
KLK2proteinsolution(0.25mg/ml)containingPhosphateBufferedSaline(pH7.4)and10%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPIVGGWECEKHSQPWQVAVYSHGWAHCGGVLVHPQWVLTAAHCLKKNSQVWLGRHNLFEPEDTGQRVPVSHSFPHPLYNMSLLKHQSLRPDEDSSHDLMLLRLSEPAKITDVVKVLGLPTQEPALGTTCYASGWGSIEPEEFLRPRSLQCVSLHLLSNDMCARAYSEKVTEFMLCAGLWTGGKDTCGGDSGGPLVCNGVLQGITSWGPEPCALPEKPAVYTKVVHYRKWIKDTIAANPHHHHHH
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
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