Prospec/TOP1 Bovine/DNA Topoisomerase-I Bovine/20µg/ENZ-656-20µg-20µg,Prospec,,Prospec,prospecbio,ProSpec-Tany,品牌试剂实验Prospec代理 prospecbio美元价/进货价,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Prospec/TOP1 Bovine/DNA Topoisomerase-I Bovine/20µg/ENZ-656-20µg-20µg

产品编号：ENZ-656-20µg-20µg

市场价：¥2600.00

场地：美国(厂家直采)

产品分类：蛋白类>酶>其它酶>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$130.00

品牌： Prospec

公司：Prospec-Tany

公司分类：

商品介绍

Catalogue number

ENZ-656

Synonyms

DNA topoisomerase 1, EC 5.99.1.2, DNA topoisomerase I, TOP1, Scl-70.

Introduction

TOP1 is an important nuclear enzyme that interconverts supercoiled DNA to the necessary topological conformations for standard DNA replication and transcription. TOP1 is the target antigen for TOP1 autoantibodies. TOP1 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are related with the existence of diffuse skin involvement and pulmonary fibrosis. In human tissues top1 enzyme is primarily synthesized as a protein with a molecular weight of 100-kDa. Most of this precursor is then proteolytically processed to a 70-kDa size, from which the TOP1 antigen has derived its name.

Description

Bovine DNA Topoisomerase-I purified from bovine tissues by proprietary chromatographic techniques.

Source

Bovine tissues.

Formulation

TOP1 is supplied at a concentration of 1mg/1ml in 16mM HEPES buffer pH-7.5, 400mM NaCl and 20% glycerol.

Immunological functions

1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checkerboard analysis of positive/negative sera panels) and immunodot test with positive/negative sera panels.

Applications

Western blot with Scleroderma positive patient sera.

Coating concentration

0.5-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.

Purity

Greater than 70% as determined by SDS-PAGE.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles.

Safety Data Sheet

SDS

Usage

Prospec"s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

品牌介绍

ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。