Prospec/VEGF (121 a.a.) Human, Sf9/Vascular Endothelial Growth Factor (121 a.a.) Human Recombinant, Sf9/2µg/CYT-200-2µg-2µg,Prospec,,Prospec,prospecbio,ProSpec-Tany,Prospec试剂产品信息 ProSpec全系列高品质细胞因子,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Prospec/VEGF (121 a.a.) Human, Sf9/Vascular Endothelial Growth Factor (121 a.a.) Human Recombinant, Sf9/2µg/CYT-200-2µg-2µg

产品编号：CYT-200-2µg-2µg

市场价：¥1000.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>生长因子>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$50.00

品牌： Prospec

公司：Prospec-Tany

公司分类：

商品介绍

Cataloguenumber

CYT-200

Synonyms

VascularendothelialgrowthfactorA,VEGF-A,VascularpermeABIlityfactor,VPF,VEGF,MGC70609.

Introduction

Vascularendothelialgrowthfactorisanimportantsignalingproteininvolvedinbothvasculogenesisandangiogenesis.Asitsnameimplies,VEGFactivityhasbeenmostlystudiedoncellsofthevascularendothelium,althoughitdoeshaveeffectsonanumberofothercelltypes(e.g.stimulationmonocyte/macrophagemigration,neurons,cancercells,kidneyepithelialcells).VEGFmediatesincreasedvascularpermeability,inducesangiogenesis,vasculogenesisandendothelialcellgrowth,promotescellmigration,andinhibitsapoptosis.Invitro,VEGFhasbeenshowntostimulateendothelialcellmitogenesisandcellmigration.VEGFisalsoavasodilatorandincreasesmicrovascularpermeabilityandwasoriginallyreferredtoasvascularpermeabilityfactor.ElevatedlevelsofthisproteinarelinkedtoPOEMSsyndrome,alsoknownasCrow-Fukasesyndrome.Mutationsinthisgenehavebeenassociatedwithproliferativeandnonproliferativediabeticretinopathy.

Description

VascularEndothelialGrowthFactor-121HumanRecombinantproducedininsectcellsasan18kDahomodimer,isaglycosylated,polypeptidechaincontaining121aminoacidsandhavingamolecularmassofapproximately36kDa.VEGF121circulatesmorefreelythanotherVEGFforms,whichbindmoretightlywithvascularheparinsulfates.TheVEGF-121ispurifiedbyproprietarychromatographictechniques.

Source

Sf9,InsectCells.

PhysicalAppearance

SterileFilteredWhitelyophilized(freeze-dried)powder.

Formulation

Theproteinwaslyophilizedfromasolutioncontaining50mMaceticacid.

Solubility

ThelyophilizedVEGF121shouldbereconstitutedin50mMaceticacidtoaconcentrationnotlowerthan50µg/ml.

Stability

LyophilizedVascularEndothelialGrowthFactor121althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionVEGF-121shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.

Purity

Greaterthan95.0%asdeterminedbySDS-PAGE.

BIOLOGicalActivity

MeasuredinacellproliferationassayusingprimaryHUVECs.TheED₅₀forthiseffectistypically2-10ng/ml.

Aminoacidsequence

APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKCDKPRR.

SafetyDataSheet

SDS

Usage

Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.

品牌介绍

ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列，专注于蛋白（重组及合成）生产研发，其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子，生长因子，激素，信号蛋白，病毒抗原等近800种重组蛋白和100多种抗体，是世界上提供蛋白品种最多的公司之一，领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。