Cataloguenumber
HOR-301
Synonyms
Glucagon,GCG,GLP1,GLP2,GRPP.
Introduction
Glucagonisanimportanthormoneinvolvedincarbohydratemetabolism.Thehormoneissynthesizedandsecretedfromalphacells(a-cells)oftheisletsofLangerhans,whicharelocatedintheendocrineportionofthepancreas.Glucagonisreleasedwhentheglucoselevelinthebloodislow(hypoglycemia),causingthelivertoconvertstoredglycogenintoglucoseandreleaseitintothebloodstream.Theactionofglucagonisthusoppositetothatofinsulin,whichinstructsthebody"scellstotakeinglucosefromthebloodintimesofsatiation.
Glucagonisbeneficialforthecultureofsomecelltypes.Ithasbeenusedinsomebiochemicalregulationstudiesofglycogenolysisinhepatocytes.IthasbeenalsobeenfoundtoinduceDNAreplicationinprimaryculturesofadultrathepatocyteswhenusedincombinationswithEGFandInsulin.Glucagonincreasesthebloodglucoseconcentrationbypromotingrapidbreakdownofliverglycogen,andalsoactstorelaxsmoothmusclesuchasthegastrointestinaltract.
Description
GlucagonHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining112aminoacids(90-180a.a.)andhavingamolecularmassof12.8kDa.
Glucagonisfusedtoa21aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
Glucagonproteinsolution(0.5mg/ml)containing20mMTris-HClbuffer(pH8.0),1mMDTT,50%glyceroland0.2MNaCl.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMKRHDEFERHAEGTFTSDVSSYLEGQAAKEFIAWLVKGRGRRDFPEEVAIVEELGRRHADGSFSDEMNTILDNLAARDFINWLIQTKITDRK.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.