Cataloguenumber
PRO-2225
Synonyms
DHLAG,HLADG,Ia-GAMMA,li,HLA-DRantigens-associatedinvariantchain,p33,Iaantigen-associatedinvariantchain,HLAclassIIhistocompatibilityantigengammachain,CD74,GammaChainOfClassIIAntigens,MHCHLA-DRGammaChain.
Introduction
CD74(HLAclassIIhistocompatibilityantigengammachainisoformb),isatransmembraneprotein(onantigenpresentingcells)whichistheinvariantchainthatchaperonesMHCclassIIdimersfromtheendoplasmicreticulumtothecellsurface.CD74isexpressedbycellsofbothTlymphocyteandBlymphocytelineages.CD74upregulatedinseveralcancersandisalsoexpressedbynonimmunecellsduringinflammation.DiseasesrelatedwithCD74includethymicepithelialneoplasmandgastroduodenitis.Amongitsrelatedsuper-pathwaysareImmuneSystemandImmuneresponseAntigenpresentationbyMHCclassII.InadditionCD74functionsascellsurfacereceptorforthecytokineMIF.
Description
CD74producedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining166aminoacids(73-232a.a.)andhavingamolecularmassof19.05kDa(MolecularsizeonSDS-PAGEwillappearatapproximately18-28kDa).CD74isexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
CD74proteinsolution(0.5mg/ml)containsphosphatebufferedsaline(pH7.4)and10%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
QQQGRLDKLTVTSQNLQLENLRMKLPKPPKPVSKMRMATPLLMQALPMGALPQGPMQNATKYGNMTEDHVMHLLQNADPLKVYPPLKGSFPENLRHLKNTMETIDWKVFESWMHHWLLFEMSRHSLEQKPTDAPPKESLELEDPSSGLGVTKQDLGPVPMHHHHHH.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
ProSpec是一家全球著名细胞因子蛋白及相关抗体生产和研发品牌。ProSpec的生产和研发工厂位于以色列,专注于蛋白(重组及合成)生产研发,其独有的细菌和哺乳动物表达和蛋白折叠技术使其能在17年内成长为国际一流的科研级蛋白供应商。目前ProSpec可以提供细胞因子,生长因子,激素,信号蛋白,病毒抗原等近800种重组蛋白和100多种抗体,是世界上提供蛋白品种最多的公司之一,领先的生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。绝大部分产品是天然成熟型蛋白,而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液,ProSpec-Tany绝大部分产品没有添加保护剂或盐,这就对工艺提出了更高的要求。同时,ProSpec-Tany绝大部分产品为冻干粉,因此易于运输和保存。